IL-4 and its polymorphism (<i>IL4</i>-589C/T) in cervical neoplasia

The transition of cervical neoplasia (CIN) to cancer occurs with the active participation IL-4, for which both pro- and antitumor effects have been shown tumors various localizations. expression cytokines is regulated at transcriptional level in promoter region gene. It has that genotype IL4 (589C/T) (rs2243250) associated development gastric breast cancer. contribution IL-4 genotypic variations CIN not yet studied. aim study was assess risk developing by presence polymorphism IL-4. object circulating neutrophils, serum genomic DNA 36 patients 20 women without dysplasia (comparison group). Using ELISA, determined neutrophil lysate serum. Phagocytic activity adhesive ability (CD11b) neutrophils were assessed. Allele-specific real-time PCR using Taq-Man probes used analyze 589C/T (rs2243250). Statistical processing carried out Statistica 13 Jamovi 1.6.5.0. As a result study, it found significantly higher than comparison group. -589C* allele gene TT are more common group (55.5%) control (25%). At same time, direct relationship established between increased indicators phagocytic number neutrophils. Analysis incidence C589T «case-control» method showed chances formation carriers -589C 3.75 (95% CI: 1.013 - 13.880, Chi-square = 4.161, p 0.042). genotype, levels HPV infection. binary logistic regression model, we demonstrated possibility differential diagnosis (χ 2 15.6, 0.001). Significant significance their combination assessed ROC-curve analysis (IL-4 neutrophils; (-589С*), 75% probability. Thus, In HPV-infected patients, can serve as marker early detection prognosis CIN.